The Potential Wound Healing Activity of Mussaenda Philippica (Rubiaceae) Methanolic Leaf Extract

This study investigated the wound-healing efficacy of Mussaenda philippica leaf extract, a plant traditionally used for wound management. Methanol-extracted leaf compounds were formulated into topical ointments at 1%, 5%, and 10% concentrations and tested on excised wounds in male mice, with 10% povidone-iodine and plain ointment serving as positive and negative controls, respectively. Phytochemical analysis identified tannins, flavonoids, and saponins-bioactive compounds associated with healing mechanisms. Acute toxicity assessments demonstrated no adverse effects, confirming the extract’s safety. The 10% formulation exhibited the most potent wound-healing activity, achieving complete epithelialization within 14 days, comparable to the 10% povidone-iodine group. The 5% concentration also showed significant efficacy, outperforming the negative control, while the 1% formulation demonstrated minimal therapeutic impact. These findings validate M. philippica’s traditional use and suggest its potential as a safe, cost-effective alternative to synthetic wound care agents. Further pharmacological and clinical studies are recommended to optimize dosage and evaluate translational applicability in humans.