Extraction & Evaluation of Biological Activity of Silver Nanoparticles Synthesized using Tabermontana Devaricata Leaf

The growing need for eco-friendly nanomaterial synthesis has accelerated interest in green chemistry approaches. This study reports the green synthesis of silver nanoparticles (AgNPs) using a hydroalcoholic leaf extract of Tabernaemontana divaricata, a plant rich in bioactive phytochemicals. The extract, obtained via Soxhlet extraction, acted as both a reducing and stabilizing agent. Nanoparticle formation was visually confirmed by a color change and further validated by UV-Visible spectroscopy, showing a surface plasmon resonance peak at 420–450 nm. Scanning Electron Microscopy (SEM) revealed well-dispersed, spherical AgNPs within a 20–50 nm size range. FTIR analysis indicated the presence of functional groups like hydroxyl, carbonyl, and amine, confirming phytochemical involvement in synthesis and stabilization. The AgNPs demonstrated exhibited efficient photocatalytic degradation of methylene blue under sunlight. These findings highlight the potential of T. divaricata-based AgNPs in biomedical and environmental applications.