Isolation of Cellulase Producing Microorganism and Characterization of Partially Purifed Enzyme

Cellulase enzymes can convert plant resources into simple sugars,cellulase enzymes are becoming more and more significant for industry. The goal of this study was to identify and investigate cellulase producing bacteria from local soli and fruit waste. We also tried to use liquid fermentation to enhance the environment so that these bacteria might produce more enzyme.To see if bacteria could degrade cellulose, they were tested on CMC agar plates. They were picked because they created distinct zones around them. Among these, two bacteria, CBM21 and CSG08, had a high capacity for cellulose degradation. It was determined that CSG08 was Pseudomonas and CBM21 was Bacillus.With an activity of 0.156 U/ml once the growing conditions were improved to include a pH of 7.0, a temperature of 400C, 5% glucose, and a 24-hour incubation period. The enzyme activity of CSG08 also increased, rising from 0.106 to 1.23U/ml.These results show that local bacteria can be useful for making enzymes for industries.Food processing, plant waste conversion, and biofuel production can all benefit from these enzymes. Changing the growing environment can also help the bacteria produce more enzyme, according to the study. (1)(2).