Investigation of Anti-Microbial Activity of Juglans Regia L. against P. Aeruginosa and B. Subtilis

Antibacterial activities of methanolic extract and ethyl acetate extract of Juglans regia . fruit husk cultivated in Kashmir were probed. Hot extraction process with methanol and ethyl acetate was carried out by employing Soxhlet apparatus method . The antimicrobial activity was calculated employing agar diffusion method. The extracts of fruit husk manifested antimicrobial activity against Pseudomonas aeruginosa as well as Bacillus subtilis along with minimum inhibitory concentration (MIC) of 100 μg/mL for Juglans regia (EA) extract against Pseudomonas aeruginosa,50 μg/mL for Juglans regia (MeOH) extract against Pseudomonas aeruginosa, 100 μg/mL for Juglans regia (EA) extract against Bacillus subtilis and 50 μg/mL for Juglans regia (MeOH) extract against Bacillus subtilis. The outcome established that the methanolic extract of Juglans regia fruit husk display antibacterial property against Pseudomonas aeruginosa and Bacillus subtilis.